MFE-280-CAS9 cell line

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MFE-280-CAS9 Order

MFE-280-CAS9 cell line
Catalog#
YC-C101-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
MFE-280-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
MFE-280-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MFE-280 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MFE-280-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name Target Name Cт Mean ΔCт
MFE-280-CAS9 Cas9 14.44739628 -1.21080685
MFE-280-CAS9 β-actin 15.65820313
MFE-280 Cas9 34.59645844 18.11198998
MFE-280 β-actin 16.48446846
Cutting Efficiency Validation
MFE-280-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the MFE-280-CAS9 stable cell pool which is electroporated by ARID2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:MFE-280
STR Info (Cell bank)
Cell Line:MFE-280
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10121012
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 7777
AMEL XXXX
TPOX 910910
CSF1PO 912912
D12S391 2222
FGA 2124
D2S1338 1920
D21S11 3030
D18S51 1318
D8S1179 1113
D3S1358 1818
D6S1043 1416
PENTAE 713
D19S433 1316
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
MFE-280-CAS9 cell line
MFE-280-CAS9
Catalog#
YC-C101-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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